The study was undertaken to investigate the impact of physico-chemical properties of soil on spore density, root colonization and distribution of arbuscular mycorrhizal (AM) fungal species associated with mulberry (Morus sp.) from ten districts of Tamil Nadu, India. The AM spore density and root colonization were high positively correlated with N (r = 0.742 and 0.785 respectively), P (r = 0.877 and 0.857 respectively), K (r = 0.944 and 0.929 respectively) and OC (r = 0.939 and 0.956 respectively), less positively correlated with EC (r = 0.018 and 0.002 respectively) and negatively correlated with pH (r = -0.381 and -0.295 respectively). Out of the native AM fungi identified, Glomus was found to be predominant followed by Acaulospora and Scutellospora. Our findings highlight the relationship between soil nutrients and AM fungi, and hence provide an insight into the potential use of AM fungi for the growth and development of mulberry.