Rough lemon is considered as the third most important citrus species of North Eastern Region of India. This rank is addressed to it not only because of its importance as a potent rootsock but also because of its nutritious, delicious and aromatic fruits. But due to highly cross pollinated nature of citrus, there is a wide variability of the species visible in the region. This variation is mainly recognised after the bearing of fruits. However, the leaf and tree characters can also be considered for identification and grouping of jambhiri species of different types. In this paper, clustering of collections was done based on leaf and tree characters. Locational diversity of rough lemon can also be observed and effect of location is also found profound from this study.