Yoghurt is a favourite dairy product for billions of people around the world and the producers constantly seek out ways of bringing new varieties for new eating occasions to be enjoyed anywhere and anytime. Food fortification is thought to be a highly effective solution and among the most cost effective public health interventions currently available. The crystalline vitamin D the carotene were fortified in yoghurt. The optimized level of fortification of vitamin D at the concentration of 1500 IU per litre of milk and carotene at the concentration of 15% per litre of milk were incorporated in yoghurt and subjected to sensory evaluation, texture analysis and microbial profile during storage up to 14 days at 5⁰C. Further, the vitamin D and carotene fortified yoghurt samples were subjected to High Performance Liquid Chromatography (HPLC) to assess the retention of vitamin D and carotene in the fortified product during storage and found satisfactory. Hence, the developed Vitamin D and carotene fortified yoghurt will address the nutritional deficiency prevalent among the public and thereby improves the nutritional status.