Journal ID : AMA-25-05-2023-12285
[This article belongs to Volume - 54, Issue - 05]
Total View : 453

Title : Development and characterization of saline tolerant mutants through in-vitro mutagenesis in rice (Oryza sativa L.)

Abstract :

The present investigation was conducted to develop mutants of rice varieties namely ADT43 and Kalinga for salt tolerance through in-vitro mutagenesis. The embryonic calli were produced and were treated with different doses of gamma irradiations and EMS concentrations and untreated calli was maintained for control. LD50 value of both the mutagen treated calli was found and the mutated calli of the two varieties were transferred to the media with optimum EC level. It was observed that the survival response of both gamma irradiated and EMS treated calli decreased under the optimum EC level. The rice cultivar ADT 43 recorded the maximum survival percentage of 32% at 30 Gy and 30% at 0.05% EMS under the optimum EC 10 dS/m, while the cultivar Kalinga recorded the maximum survival percentage of 28% at 20 Gy and 15% at 0.01% EMS under the optimum EC 10.3 dS/m. The in-vitro regenerated plantlets of ADT 43 and Kalinga obtained after in-vitro mutation were screened at the optimum EC level of the respective cultivars. After critical evaluation, the survived salt tolerant plants of M1 were forwarded to M2 where selection was made and identified six mutant lines viz., A-M2-15-1, A-M2-15-2, A-M2-15, K-M2-5-1, K-M2-5-2 and K-M2-5-3 from ADT 43 and Kalinga which exhibited better performance in biometrical observations and biochemical parameters like proline content, chlorophyll content, Na+/K+ ratio, APX activity, and SOD activity.

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