Journal ID : AMA-07-11-2022-11793
[This article belongs to Volume - 53, Issue - 11]
Total View : 444

Title : Developing Hapten Conjugates For Imidacloprid Immunoassay

Abstract :

Pesticide residues are determined using sophisticated equipment such as UHPLC, LCMS, GC, and GCMS, which necessitate high operating costs, a controlled atmosphere for instrument housing, and technical personnel for handling. The field of immunoassay has made it possible to determine pesticide residues in food products in situ without the assistance of technical personnel. In order to establish an immunoassay approach, it is necessary to synthesize hapten and conjugate it with a carrier protein for generating an immunological response in any animal system. Therefore, the method for synthesizing imidacloprid hapten and the covalent linkage with carrier proteins such as Bovine Serum Albumin (BSA) were explored in this work. Thin-layer chromatography analysis of synthesized hapten revealed that its Rf value was distinct from that of the imidacloprid standard (0.98) and hapten (0.2). The production of imidacloprid hapten was subsequently confirmed by LCMS and 1HNMR. The generated hapten was conjugated with BSA using the active carboxyl ester technique for further use as an antigen.

Full article