An investigation was conducted at Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST–Jammu) to study the bio-intensification possibilities in different cropping systems. The experiment was laid out in randomized block design (factorial) with 15 treatments and three replications. Among different crop bio-intensification systems, Wheat + Mustard (6:1) – Moongbean– Maize (Bed) + Cowpea (B) recorded higher Wheat equivalent yield (WEY) (142.64 q/ha and 144.86 q/ha), crop profitability (Rs 337/ha /day and Rs 352/ha/day), system profitability (Rs 304/ha/day and Rs. 324/ha/day), production efficiency (43.36 kg/ha/day and 43.12 kg/ha/day) during both years of experimentation. With regard to microbial population, higher population of bacteria (13.55x106 cfu/g and 15.92 x 106 cfu/g), fungi (20.17 x 106 cfu/g and 22.18 x 106 cfu/g) and actinomycetes (18.27x106 cfu/g and 20.10 × 106 cfu/g) was recorded in Wheat (FIRB)+Lentil (B) – Moongbean – Brown Manuring +Rice (F) + Moongbean (Bed) as compared to rest of the treatments during 1st and 2nd year of experimentation. Amongst the nutrient management practices, 100% inorganic nutrient management practice recorded higher WEY (12585 kg/ha and 12740 kg/ha), crop profitability (Rs 360/ha/day and Rs 374/ha/day), system profitability (Rs 314/ha/day and Rs 332/ha/day), production efficiency (39.58 kg/ha/day and 39.32 kg/ha/day) during both years of experimentation. Thus, application of 100% inorganic nutrient management practice in Wheat + Mustard (6:1) – Moongbean– Maize (Bed)+Cowpea(B) crop bio-intensification system has yielded maximum WEY of 127.40 q/ ha with net returns (Rs 121033) and B:C ratio of 1.90.