There is a paucity of information available on the practicality, suitability, and profitability of in vitro raised plants of clonal apple rootstocks as compared to field-multiplied plants through traditional methods. The present investigation was carried out at the Plant Tissue Culture laboratory of the School of Biotechnology (SBT) and the Tissue Culture laboratory of the Advanced Centre for Horticulture Research (ACHR), Udheywalla, SKUAST-Jammu, Main Campus, Chatha. Results indicated that the unit cost for in vitro raised plantlets from explants excised from a 2-3 year-old mother block of clonal apple rootstock MM 106 was estimated to be Rs. 44.56, which included Rs. 41.38 and Rs. 3.18 at the tissue culture production phase and greenhouse production phase, respectively, whereas, in MM 111 clonal rootstock, the unit cost for in vitro raised plantlets was estimated to be Rs. 36.95, which included Rs. 34.29 and Rs. Moreover, as per the general practices in the literature, the estimated production cost of an ex vitro seedling is Rs. 150.