A total of 120 dogs of different breeds were presented to OPD of Veterinary Clinical Complex over a period from May 2023 to May 2024, with a wide range of symptoms as abnormal dark color urine, fever, inappetence to anorexia, vomition, drooling of saliva and stiffness in neck and limbs. Most of the dogs were dull and depressed. Further, clinical examination depicted pale mucous membrane, variable rectal temperature (103.6⁰F to 106.4 ⁰F) and the presence of ticks on the body. Hematological findings revealed decreased RBC as well as PCV levels along with slight neutrophilia. Blood smear examination revealed Babesia gibsoni in the erythrocyte. Dogs infected with canine babesiosis were grouped in 3 treatment groups i.e., Group- 1, 2, 3. Sixty positive cases of canine babesiosis were treated with different combination of drugs. In Group 1, dogs (n=20) were treated with intramuscular administration of Imidocarb diapropionate @ 6.6 mg/ kg body weight repeated after 2 weeks. In Group 2, dogs (n=20) were treated with intramuscular administration of Diminazine aceturate @ 3.5mg/kg body weight along with PO administration of Tab. Doxycycline @ 10 mg/ kg body weight for 21 days. Meanwhile in Group 3, dogs (n=20) were treated with Tab. Clindamycin @ 22 mg/kg body weight OD + Doxycycline @ 5 mg/kg body weight OD + Metronidazole @ 20 mg/kg body weight OD. Fluid therapy along with supportive medications was also given in all the three groups. Results of this study clearly suggested that a group 3 therapeutic protocol is better in comparison to group 1 and 2.