Traumatic reticulo-pericarditis (TRP) is a serious and economically important condition in bovines, particularly under smallholder farming systems common in Asia and Africa. The present study compared the therapeutic efficacy of intrapericardial infiltration of autologous platelet-rich plasma (PRP) and dimethyl sulfoxide (DMSO) following pericardiostomy in bovines affected with TRP. Eighteen bovines diagnosed ultrasonographically with TRP were randomly divided into three groups (n = 6 each). All animals underwent ultrasound-guided pericardiostomy and standard medical management. Group A received intrapericardial normal saline (control), Group B received autologous PRP, and Group C received 4% DMSO for three consecutive days. Clinical parameters, ultrasonographic findings, cardiac biomarkers (cTnI and NT-proBNP), and oxidative stress markers (MDA, SOD, GSH, TAC) were evaluated on day 0 and day 5. PRP-treated animals demonstrated superior clinical recovery, faster normalization of heart rate, significant reductions in cardiac biomarkers, and improved antioxidant enzyme activity compared with DMSO-treated and control animals. The findings indicate that PRP provides enhanced cardioprotective and regenerative effects in bovine TRP, offering a promising adjunct to surgical management under field conditions.