A total of 160 epiphytic yeast strains associated with various genotypes of chilli (Capsicum spp.) cultivated in Meghalaya, India, were isolated and subjected to assessment for their antagonistic potential against a pathogenic strain of Colletotrichum truncatum, causal agent of fruit rot in chilli. Notable variations were observed in the inhibition of mycelial growth of the pathogen through a dual culture assay, with mean inhibition per cent ranging from 31.55% to 64.81%.Four distinct yeast isolates, namely YE12 (identified as Saturnispora sylvae), YE46 (identified as Meyerozyma guilliermondii), YE64 (identified as Rhodotorula paludigena), and YE127 (also identified as Meyerozyma guilliermondii), were chosen for subsequent evaluation in controlled pot experiments as well as under field conditions. Notably, the synergistic application of these efficient yeast isolates exhibited superior outcomes compared to their individual applications. Among all the single and combined treatments, the consortium comprising the four yeast isolates, i.e., YE17+YE46+YE64+YE127, demonstrated the highest reduction in disease incidence, with a substantial decrease of 77.85% in the pot experiment and 64.57% reduction in the field experiment, respectively. Furthermore, the treatment involving the combination of Saturnispora silvae, Meyerozyma guilliermondii, Rhodotorula paludigena, and Meyerozyma guilliermondii exhibited notable improvements in both plant health and yield attributes across both experimental conditions.