Aloe Vera plant is reliable and has potential to cure bacterial infection in contrast to conventional antibiotics. Aloe Vera gel can be used to prevent the risk of transmission of virus, bacteria or fungi through hand contamination. They are used more in health sector and in the pharmaceutical industry to avoid contamination among people. This renewed interest comes the fact that medicinal plants represent an inexhaustible source of natural bioactive substances and compounds. Aloe Vera gel at higher concentration showed anti-bacterial property. Anti-microbial activity of the Aloe Vera gel was tested by the Agar well diffusion method (AWDT). Staphylococcus aureus, klebsiella pneumoniae, Escherichia coli, Bacillus cereus and Candida albicans strains were used in this work. For Hydro-alcoholic solution, the highest inhibition diameter (40 mm) is observed with the Staphylococcus aureus strain while the lowest inhibition diameter (18 mm) is recorded in Escherichia coli. For the Aloe Vera gel; the highest inhibition diameter (30 mm) was observed against the Bacillus strain while no effect on the Escherichia coli, Staphylococcus aureus, klebsiella and C. Albicans strains is recorded. For the commercialized gel; Inhibition diameter (14 mm) was observed against the klebsiella strain (while on the rest of the tested strains). The aim of this study were to determine the antimicrobial and inhibitory activities of various concentrations of Aloe Vera Gel (AVG) against pathogenic bacteria and made a comparaison with hydro-alcohol solution (HAS).