The present study was aimed to assess the general combining ability of parents and specific combining ability of their crosses for yield and quality traits. Nine inbred lines were crossed with three testers using line × tester mating design. Nine lines, three testers and 27 F1s were evaluated along with two checks in this study for grain yield per plant, grain protein content, tryptophan content and lysine content during Rabi 2021-22 and Spring 2022. Total 41 genotypes (27 crosses, 9 lines, 3 testers and 2 checks) were evaluated in Randomized Block Design with three replications at experimental farm of BAC, Sabour. Based on general combining ability analysis parents L4, L6, L9 were found as good general combiner for grain yield & quality traits in rabi season. L1, L2 and L3 were accompanied with significant GCA in desired direction for tryptophan and lysine content in spring season. Among the crosses L2 × T1, L7× T3 and L3× T1, L9 × T3 exhibited highly significant and desirable SCA effects for grain yield per plant in rabi and spring season respectively. L3× T3 possessed highly significant SCA effects for protein, tryptophan and lysine content in spring season. Therefore, these crosses can be utilized for developing high yielding good quality hybrid varieties in maize. There was preponderance of non-additive gene action in the inheritance of all the traits.