This research work focuses on chemical modification of jute substrate and development of a multifunctional treatment which improves several drawbacks of jute fabric. The assembly of sodium hydroxide, sodium carbonate and sodium sulphite was applied on jute fabric through exhaust method. The results revealed decrease in bending length, flexural rigidity and drape coefficient pointing towards increase in softness and drape of the jute fabric. The colony forming units of Gram +ve bacteria (Staphylococcus aureus) and Gram -ve bacteria (Escherichia coli) decreased. Additionally, there was decrease in transmission of both UV – A and UV – B rays. The surface morphology was studied by scanning electron microscopy. The results suggested that chemical modification of jute substrate is multifunctional and results in improving both physical and functional properties of the fabric.