Journal ID : AMA-29-09-2024-13247
[This article belongs to Volume - 55, Issue - 10]
Total View : 431

Title : Characterization of Rice (Oryza sativa L.) Genotypes based on Badh2 Gene Specific Marker and its Effect on Aroma Intensity

Abstract :

One of rice's most significant quality attributes is fragrance, and the gene badh2 (betaine aldehyde dehydrogenase) is responsible for this phenotype. In order to meet the growing demand for fragrant rice, one of the main goals of the present rice breeding programmes is to improve fragrant rice. A dominant Badh2 allele encoding betaine aldehyde dehydrogenase inhibits the synthesis of 2-AP, and the recessive allele induces 2-AP formation. The present investigation was performed to evaluate the genotype having functional Badh2 gene and non-functional Badh2 gene and its effect on aroma intensity which was tested by 1.7 % KOH a sensory method. In this study, those genotypes which gave approx. 355 bp band on Badh2 gene specific marker have trace or no aroma. Those genotypes that gave approx. 257 bp band with Badh2 gene specific marker had aroma in high intensity and those genotypes that gave both 355 and 257 bp band had aroma but less in compare to those genotypes that gave 257 bp band on Badh2 gene specific marker. Change in functionality of Badh2 causes change in aroma intensity and its presence.

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