Nanotechnology, a rapidly advancing field, enables the reduction of particle size to below 100 nm, thereby significantly increasing surface area and enhancing mineral bioavailability. In animal nutrition, this technology is primarily applied to the development of nanominerals - particularly trace minerals with inherently low absorption efficiency. Nanominerals not only improve bioavailability but also minimize intestinal mineral antagonism, leading to reduced excretion and lower environmental contamination. In this context, copper, cobalt, and zinc nanominerals were synthesized using a physical method involving a planetary ball mill. The synthesized nanominerals were characterized for particle size, morphology, surface charge (zeta potential), crystalline structure, and functional groups. The average particle sizes of the synthesized Nano Cu, Nano Co, and Nano Zn, as determined by particle size analysis, were 51.33 nm, 74.07 nm, and 77.40 nm, respectively. The corresponding zeta potentials were -24.73 mV (Nano Cu), -18.50 mV (Nano Co), and -22.10 mV (Nano Zn), indicating good colloidal stability. Transmission Electron Microscopy (TEM) revealed that Nano Cu and Nano Co exhibited a spherical morphology, while Nano Zn displayed a hexagonal shape. The yield of nanominerals was approximately 95% for all the three minerals. The XRD analysis verified that the synthesized nanominerals were pure, as no additional characteristic peaks were observed apart from those corresponding to their respective elements. FT-IR findings confirm the successful formation of metal nanoparticles with distinct surface properties. Furthermore, an in vitro cytotoxicity assessment revealed that the viability of Vero cells in MTT assay was more than 70 % for all the three nanominerals at concentrations varying from 6.25 – 100 μg/ml. Hence, Nano Cu, Nano Co and Nano Zn can safely be incorporated in the livestock feed up to the concentration of 100ppm.