Greenhouse is known as one of the media commonly used for the planting process with a room control system. In accordance with its work objectives, a greenhouse was formed to be able to produce a room whose environmental conditions can be controlled properly. The use of aluminum-cotton as a greenhouse cover material is expected to be an alternative to better maintain temperatures in the greenhouse and also more economical. Lately the improvement of cotton features in various fields and utilization has also begun to be done. In this study, variations in fabrication layers were made to determine the effects of layers and also the appropriate type of fabric used as thermal insulators. There were nine types of aluminum cotton fabric layers as the variation. As a testing tool, the heating simulator system was self-designed using wood and aluminum plates. The analysis conducted in this study was the temperature changes that occur in the control room after 5 and 10 minutes of testing. The results of analysis showed that variations in fabrication layers provided a significant effect on material weight, controlled room temperature after 5 minutes, and controlled room temperature after 10 minutes. The most suitable samples to be used as thermal insulators based on tests in this study were samples A and E, which showed that there was a significant increase in temperature after 5 and 10 minutes of processing: 5ºC and 6ºC for sample A, and 1.67ºC and 2ºC for samples E.