There is a diverse variety of pumpkin types found in nature, and their potential as a source of pro-vitamin A can be evaluated with the goal of using them in traditional plant breeding or biofortification projects with the goal of increasing the beta-carotene content. The objective of the study was to determine the β-carotene contents in locally available varieties and their hybrids (L x T fashion) of fully ripened pumpkins to verify their use in further breeding program as parents. High Performance Liquid Chromatography were used to identify and quantify the β-carotene contents in. In this study carotene content in pumpkin fruit flesh varied from 4.70 µg per g (L2) to 24.57 µg per g (L4) in lines whereas in testers it ranged from 7.73 µg per g (T3) to 11.59 µg per g (T2). Among the 18 crosses of study, the lowest and highest values of β carotene content recorded by the crosses L4 x T1 (2.54 µg per g) and L3 x T2 (89.60 µg per g) respectively. In total, six hybrids exceeded the parents in their β carotene content above the mean value (20.57 µg per g). The highest carotene content recorded by L3 x T2 (89.60 µg per g) followed by L1 x T1 (40.32 µg per g) and L2 x T2 (28.06 µg per g).