The growth of the Scenedesmus spinosus microalgae was evaluated in four culture mediums: Z8, Watanabe, Bristol and BG-11, using a pilot raceway photobioreactor. The reactor used had a culture volume of 1.2 m3, it was built of fiberglass and covered with a transparent polycarbonate lid on top to avoid contamination. Turbidity, total solids, solar irradiation, and temperature were measured for a period of 28 days. Biomass growth was evaluated through the change in turbidity over time and gravimetry tests, for the four analyzed culture mediums. The culture medium showing the highest biomass yield over the time corresponded to the Z8 medium, closely followed by Watanabe, while Bristol and BG-11 generated the lowest biomass increment. The highest productivity value corresponded to 55.7 g m-3d-1 with Z8 medium while the lower biomass productivity figure corresponded to 5.6 g m-3d-1 with Bristol medium.