Date pits are the waste product of several date processing industries, and are either discarded or partially incorporated into animal feed. A number of studies have revealed their richness in various interesting biochemical and mineral substances. Our research focuses on the methanolic extraction of three varieties of Phoenix dactylifera L. date pits: DEGLAT-NOR, MECH-DEGLA and HMIRA. Chemical analyses revealed a high concentration of phenolic compounds (31.60 mg GAE/g extract) for the HMIRA variety, 28.77 mg GAE/g extract for DEGLAT-NOR and 20.39 mg GAE/g extract for MECH-DEGLAand flavonoids 20.841 mg CQE/g dry extract for the HMIRA variety, 15. 711 mg CQE/g dry extract for DEGLAT-NOR, 13.333 mg CQE/g dry extract for MECH-DEGLA and the results indicate a high antioxidant activity with an IC50 of 1.64 mg/ml for the HMIRA variety, DEGLAT-NOR confirms an IC50 which is 1.835 mg/ml and IC50 which is 2.017 mg/ml for the MECH-DEGLA variety. HMIRA date stones have a higher antioxidant power than the DEGLET NOUR and MECH-DEGLA varieties. Its richness in polyphenols and flavonoids exceeds that of the other two varieties. Antimicrobial activity was determined on four bacterial strains (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus sublilis, Escherichia coli), using the disc diffusion method. The results indicate that the methanolic extract of date stones of three varieties has antibacterial activity at different concentrations.