Bioinformatic Annotation of Triticum aestivum 1A chromosome from assembled whole genome is not yet complete and lacks participatory contributions to provide more details on its physical map and analyze SSRs (mono-,.. hexanucleotide) statistical distributions. We present, in our work, a script in python language to pre-process the assembled chromosomal sequences and the Krait program for the structural annotation of chromosome 1A to show the possible distributions of all the SSR types as well as the primers likely to be used for PCR sequence amplification. Our results show that the python script is a good tool for genomic data pre-processing. Moreover, the dimeric motifs AG, AT and AC are the most abundant in 1A chromosome and are followed by mononucleotide and trinucleotide SSRs C and AAG respectively. The occurrence of tetra repeats on 1A chromosome is 4219 sequences (72716 bp) corresponding to 8.43% of the total 1A chromosome. The relative abundance and density are respectively 7.15 loci/Mb and 123.16bp/Mb. The highest distribution was observed for the trinucleotide motifs (n = 14,903), characterized by Ra = 25.24 and Rd = 514.94 bp/Mb. Within this category (Trinucleotide), the motifs AAG and AGG were the most dominant with values of 2,987 and 2,431, respectively. The Triticum aestivum 1A chromosome shows all of the SSR types. We observed a polymorphic and unevenly SSRs distribution along the 1A chromosome.