Extinction of fossil fuel and thriving energy consumption pave the way for development of sustainable bioethanol as a top-tier biofuel. Bioethanol can be used in pure form or added to gasoline as gasohol and can be used as octane enhancer in other transportation fuel. Our study highlighted bioethanol production from lignocellulosic biomass through syngas fermentation conducted in a bioreactor. The conventional yeast Saccharomyces cerevisiae used in the bioprocess technology depicted 8% tolerance towards ethanol and produced 1.40% of bioethanol through syngas (60% CO+ 20% CO2+ 25% H2+ 5% CH4) fermentation at 37oC in 15 days. Bioethanol quantification was established by GC analysis. Further, optimization of different bio-parameters for enhanced bioethanol production is highly essential to scale-up the process, which may reduce the dependency on fossil fuel by contributing a green and sustainable energy.