Mungbean (Vigna radiata (L.) Wilczek) is a very ancient annual crop and India is the world’s largest producer of mungbean with approximately 30% of global output of 5.3 million t. Various diseases of mungbean are anthracnose, rust, cercospora leaf spot, powdery mildew, stem necrosis, dry root rot, leaf crinkle and yellow mosaic disease (MYMV). Out of all diseases, MYMV disease is one of the most prevalent and destructive diseases. MYMV a member of Geminiviridae family and transmitted by whitefly (Bemisia tabaci Genn) in persistent manner. Ten mungbean genotypes were screened against MYMV diseases (MH 1801, MH 1850, MH 1851, MH 318, MH 215, MH 125, MH 1811, MH 1870, MH 1814 and SML 1082). The current study was aimed to know about changes in concentrations of biochemical parameters (chlorophyll content, carotenoids, Orthodihydroxy phenol, phenol content, flavanol and total soluble sugar) at different stages of crop infected by MYMV and disease severity. The disease severity and biochemical changes were estimated at weekly and fortnight interval respectively. The chlorophyll content and carotenoid content were higher at 25 DAS and 40 DAS but from 40 DAS to 55 DAS chlorophyll content was decreasing. Higher total phenol content estimated at initial stage, i.e. at 25 DAS and 40 DAS. It decreased at 40 DAS and 55 DAS. However, Orthodihydroxy phenol and flavanol content was observed decreasing gradually from 25 DAS to 55 DAS. Maximum phenol content and flavanol content was recorded in MH 1851 followed by MH 215.