Bio-pesticide or Biological pesticides are derived from natural materials such as animals, plants, bacteria, and certain minerals. It is less toxic and reduces the pollution problems caused by conventional pesticides. The present study studied biochemical changes in cow urine and dung-based six (T1, T2, T3, T4, T5 and T6) different bio-pesticide formulations and their efficacy on cattle tick control. The results revealed that N2, CP, Urea, Uric acid, hippuric acid and creatinine increased significantly over fermentation. In tick control, results revealed highest tick mortality (%) in T6 (53.25±0.96) as compared to T5 (46.08±0.96), T4(42.26±0.96), T3(33.44±0.96), T2(28.41±0.96) and T1(22.77±0.96), respectively. However, the cow urine and dung-based formulations had a significantly (P<0.01) higher effect on tick mortality (42.54±0.51%) as compared to heifers urine and dung-based formulations.