Chronic anti-inflammatory diseases as well as rheumatoid arthritis exist as one of the leading health complications globally. At current, while various synthetic drugs are prominent in the market however their prolonged use possibly will cause austere adverse effects on long-term usage including gastrointestinal bleeding besides peptic ulcers. Therefore around is a necessity to develop a novel anti-inflammatory agent having fewer side effects. Exploration aimed at safe in addition to effective anti-inflammatory agents has been given precedence in scientific research in herbal system of medicine. Among the natural resources one of the plant is Berberis aristata which is conventionally prescribed to treat wounds, diabetes, inflammations as well as jaundice. Our aim is to explore the therapeutic potential of biologically active constituents from Berberis aristata DC which can be serving as possible sources for new analgesic drugs.