Flowcytometry-based sorting of X-bearing sperms helped to produce calves of desirable sex calves and was successfully used in the livestock industry worldwide. In India, the usage of this technique is minimal, and there is little information about the success rate of sex-sorting technology. We examined the quality of commercially procured sex-sorted X-bearing sperms in this work by assessing X and Y sperm population per straw, live and dead percentage, acrosomal integrity, Sperm Chromatin Structure Assay (SCSA), (Reactive Oxygen Species) ROS / OS, Osteopontin (OPN), and Ubiquitin (UBI) expression. Further, we also assessed the reproductive outcome of sex-sorted bovine semen in crossbred cows. Our data showed no significant difference in acrosomal integrity, oxidative stress, and expression of OPN and UBI between frozen-thawed unsorted crossbred and commercially procured sex-sorted bull semen. However, the DNA Fragmentation index (DFI) was significantly lower in the commercially procured sex-sorted frozen-thawed semen. Interestingly, our data suggested lower conception rates in synchronized cows artificially inseminated commercially procured sex-sorted semen compared to unsorted crossbred bull semen. Despite the lower conception rate, all the eight pregnant cows inseminated with commercially procured sorted bull semen delivered only female calves. Our study indicates the usefulness of commercial sex-sorted semen in field conditions to enhance the reproductive outcome in crossbred cows in India.