Assessment of leveraging impacts of chelated micronutrients, growth regulator and biofertilizers on biochemical characters of pomegranate (Punica granatum L.) cv. Bhagwa” was carried out on experimental fruit orchard of Department of Horticulture, VNMKV., Parbhani, during Mrig bahar in 2018-19 and 2019-20. This experiment was laid out in Factorial RBD, which is replicated twice with two factors i.e. Factor A: methods of plant propagation (P) i.e. P1: Air layered plants, P2: Tissue cultured plants and Factor B: different nutrients concentrations i.e. N1: Chelated micronutrients (CM.) @ 3.0 g/lit, N2: CM. @ 4.0 g/lit, N3: GA3 @ 75 ppm, N4: GA3 @ 100 ppm, N5: Biomix @ 0.3%, N6: Biomix @ 0.4%, N7: CM. @ 3.0 g/lit and Biomix 0.3%, N8: CM. @ 3.0 g/lit and GA3 75 ppm and Biomix 0.3%, N9: CM. @ 3.0 g/lit and GA3 75 ppm, N10: GA3 75 ppm and Biomix 0.3%, N11: Control. The result revealed that biochemical characters of fruits regarding TSS, total sugars, reducing sugars, ascorbic acid and anthocyanin content increases with application of chelated micron. @ 3.0 g/l + GA3 @ 75 ppm + Biomix @ 0.3% with interaction of tissue cultured plants. Whereas, acidity of fruit is reduced due to combined application of chelated micron. @ 3.0 g/l + GA3 @ 75 ppm + Biomix @ 0.3% with interaction of tissue cultured plants. This foliar application has produced significantly superior effect on biochemical parameters.