Journal ID : AMA-14-12-2024-13349
[This article belongs to Volume - 55, Issue - 12]
Total View : 477

Title : Assessment of efficiency of microbial production of stovers through in vitro gas production parameters

Abstract :

This study was performed to determine the nutritive value of sorghum (PKV 801- 1 and PKV 801- 4) and pearl millet stovers (ICMV 155 (1H), ICMV 155 (2H), ICMV 221 (1H), ICMV 221 (2H), Jakarana, Wrajpop, Sosat C 88 and Sadore local) which were grown in ICRISAT, Patancheru using in vitro gas production test. The in vitro samples were dried and ground in Wiley mill using a medium mesh of 1 mm and the 200 mg of ground samples were incubated in triplicate in calibrated gas syringe at 390C in waterbath. Rumen inoculum was replaced by Fecal Inoculum on microbial N equivalent for in vitro gas production technique. Fresh fecal sample (FI) for in vitro gas production test was collected from two adult bullocks of Ongole breed of similar sex, age and weight, kept exclusively on stover feeding. The volume of in vitro gas production of stovers was measured at 0, 3, 6,9,12,24,48,72 and 96 hr of incubation. Gas production data were fitted to the exponential model P= a+b (1-e-ct) for calculating the kinetics of gas production. The partitioning factor (PF) was also estimated from incubation residues which were refluxed with neutral detergent fiber solution. In vitro efficiency of microbial production (EMP) was also calculated from the amount of substrate truly degraded (TSD) and gas volume. Significantly (p<0.05) the highest IVOMD was observed for stover ICMV 221 (1H). PF value at half time of gas production was ranged from 3.32 to 6.04 for stovers. The amount of organic matter truly degraded ranged from 50.7 to 109.30 mg / 200 mg substrate incubated with an average value of 66.3 mg/ 200 mg substrate observed for stovers. The lowest, time to half maximal gas production (t1/2) volume of 9.3 ml was observed in ICMV 221 (1H) variety of stover and the substrate that produces less gas volume and higher in amount of substrate truly degraded had higher feed intake. To confirm the above findings, an in vivo experiment was conducted on Deccani sheep to know the nutritive value of the stovers and resulted that the high voluntary feed intake was observed in the group fed with the stover ICMV 221 (1H). To conclude, the data related to the kinetic parameters, TSD and EMP of stovers, supports the fact that the stover variety ICMV 221 (2H) was superior than other stover varieties evaluated and had a promising future for feeding ruminants.

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