Food environments improve the health and nutrition benefits of populations. Rapidly changing food systems, production and distribution patterns stress out to leverage the role of agriculture for nutrition. This requires enquiring the knowledge of farmers in both the fields of agriculture and nutrition for appropriate decision-making. The present study attempted to investigate Agri-Nutrition (A2N) knowledge level of 360 farmers from two agro-climatic regions of Uttar Pradesh and Telangana villages with a reliable and valid instrument development based on psychometric principles. The items with difficulty index (20-80), discrimination index (>0.2), point-biserial correlation (>0.2) were selected and administered to final sample. The findings indicate targeted efforts of providing nutrition education in model villages contributed to improved knowledge among farmers, which in turn positively affect nutrition security in the study area.