A study was under taken to find out the antioxidant levels in oviductal secretion of buffaloes reared under tropical climatic conditions. Oviducts, along with ovaries, of apparently healthy buffaloes were obtained from slaughter house. The stage of estrous cycle was determined as either luteal or non-luteal based on the ovarian structures. The oviduct was dissected into isthmus and ampulla and fluid was collected from both the regions by compressing with glass slides. The extract was centrifuged at 3000 rpm for 30 min and the supernatant was centrifuged again at 6000 rpm for 30 min and the supernatant was collected. The samples were analyzed for total protein, super oxide dismutase (SOD), catalase and reduced glutathione. The total protein concentration in oviductal fluid was lower during the luteal phase compared to non-luteal phase of the oestrous cycle. Significantly (P<0.05) higher concentration of proteins was observed in ampullary fluid than the isthmic fluid irrespective of the phase of oestrous cycle. There was no significant difference in SOD concentrations between isthmic and ampullary oviductal fluids. The non-luteal isthmic fluid showed higher activity of catalase and reduced glutathine compared to other groups. It was inferred antioxidant concentrations in oviductal fluid showed significant variations with both the stage of oestrous cycle and the region of oviduct.