Launaea acanthoclada is a medicinal plant used to treat inflammations, digestive disorders and wound infections. The aim of our study was to assess antioxidant, anticoagulant, antithrombotic activities and analgesic effects of this plant extracts. The total phenols and flavonoids content of the different extracts were determined using Folin-Ciocalteu and Aluminum chloride colorimetric assays. Antioxidant activity was performed using several methods. The anticoagulant and antithrombotic activity was tested on human blood. To evaluate analgesic activity, the crude extract was subjected to acetic acid-induced writhing test. Crude extract (CrE) and ethyl acetate extract (EAE) showed a potent activity in scavenging DPPH (IC50 of 9.14 ± 1.88 and 44.3 ± 0.85 µg/mL). EAE, CrE, butanol (BuE) and hexane (HeE) fractions displayed the greater activity (> 80%) in inhibition of β-carotene oxidation. However, EAE had an IC50 of 0.87 ± 0.02 mg/mL in hydroxyl radical scavenging assay. Aqueous fraction (AqE) and EAE showed a significant percentage of clot lysis (35.15 and 30.08%). CrE, HeE and EAE inhibit blood clotting even after 3 h. In addition, CrE induced significant antinociceptive activity of 50 and 57.28% at doses of 150 and 300 mg/Kg, respectively. Our findings revealed that L. acanthoclada could be used in the development of new natural medicines.