This study evaluates the antibacterial activity of six nativeCentaurea species: C. sphaerocephala, C. tougourensis, C. napifolia, C. fragilis, C. papposa, C. solstitialis, and C. dimorpha. Three types of extracts: dichloromethane, ethyl acetate, and n-butanol, were tested for their ability to inhibit bacterial growth using the solid medium disk diffusion method at four different concentrations. The results confirm that extracts, particularly those derived from ethyl acetate, exhibit significant antibacterial activity against both Gram-positive and Gram-negative bacteria. Notably, C. papposa and C. napifolia demonstrated high efficacy against Staphylococcus aureus and Pseudomonas aeruginosa, suggesting their potential as natural antimicrobial agents. Additionally, the butanolic extract of C. papposa displayed no significant acute toxicity at a dose of 2000 mg/kg, indicating safety for further pharmacological research. This aligns with existing literature on Centaurea species, reinforcing their low toxicity potential in therapeutic applications. Histological findings revealed notable effects of the butanolic extract on the liver and lungs, highlighting the need for further investigation into the extract's safety profile and mechanisms of action. Future studies should focus on identifying the specific bioactive compounds responsible for the observed effects, enhancing our understanding of their therapeutic implications and ensuring safety and efficacy in clinical contexts.