Sesame (Sesamum indicum L.) is one of the important oilseed crop which is widely cultivated in tropical and subtropical areas of Asia, Africa and South America since ancient times. Present study was carried out to evaluate the phenotypic variability in sesame genotypes from different parts of West Bengal and some major sesame growing states of India along with eight genotypes from USA, Bulgaria and Bangladesh, using 12 quantitative traits. Genotypes like CUMS 17, CUHY 57, MT 75, Atghara, RT 351 were to some extend better performed than the check variety TKG 22 and GT 10 regarding yield and other yield attributing traits in both locations (Ranaghat and Baruipur) At both genotypic and phenotypic level, seed yield per plant showed significant positive correlations with 1000 seed weight, seeds per capsule and capsules per plant and significant negative correlation with days to maturity, which suggests that seed production is mostly depends on these mentioned characters whereas early maturity depends on early flowering. Highest oil content was observed in TKG-352(53.13) followed by EC 96, CUMS 17, RT 351, Shekhar, GT 10 those recorded > 48% oil contents. Three genotypes namely AT 306, RT 351 and HT 2 recorded higher value of soluble protein content of > 17 %. In cluster analysis genotypes grouped into various clusters exposed that there was no relationship between geographical distribution and genetic diversity. These results have an important suggestion for sesame genotypes agro-morphological characterization, evaluation and improvement in future.