Journal ID : AMA-11-04-2022-11297
[This article belongs to Volume - 53, Issue - 04]
Total View : 453

Title : An efficient in vitro regeneration protocol in guava cultivars (Hisar Safeda and Hisar surkha) using nodal and shoot tip explants

Abstract :

Guava being important cash crop grown in India, but the main technological hurdle is the good quality planting material because nowadays guava production is seriously hampered by wilting, root-knot nematodes and lack of cultivars. So, to provide true mother type plant which is free from disease and high productivity planting material, micro-propagation is only alternative and feasible tool, but micro-propagation in guava again faces problem of browning and microbial combination. So, the main aim of the study was to develop rapid and reproducible regeneration protocol for hybrid guava cvs to provide goods plants on large scale. MSM (Murashige and Skoog medium) and WPM (Woody plant medium) fortified with various combination of different plant growth regulators were used and found that WPM + 2.5 ppm BAP + 0.5 ppm IAA showed higher per cent shoot induction in nodal (51.70%) and shoot tip (52.29%) explants with 2.24 and 1.82 shoots/explants, respectively in cultivar Hisar Safeda. In Hisar Surkha, nodal explants revealed 53.81% and shoot tip explants 53.70 % shoot induction on WPM + 2.5 ppm BAP) + 0.2 ppm NAA with average of 2.04 and 1.32 shoots/explants, respectively. The in vitro regenerated shoots (micro shoots containing at least 3-4 leaves) were transplanted on different rooting medium but ½ strength MS medium + 2.0 ppm of IBA was found most effective per cent root induction of 71.78 % and 66.66% in Hisar Safeda and Hisar Surkha, respectively. These plantlets thus formed were grown on various combinations of sand, soil, vermiculite, farm yard manure and cocopeat for hardening and the medium having vermicullite and cocopeat (1:1) was found most effective with maximum 50-60% survival of plant after transplantation.

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