This proposal study is to analyze the performance of two different types of hydro-pneumatic suspension struts for an agricultural truck. The nonlinear dynamical models of two different types of hydro-pneumatic suspension struts with two oil chambers and one gas chamber (Model 1) and with three oil chambers and one gas chamber (Model 2) are set up to determine their nonlinear forces in the vertical direction. A quarter–vehicle mathematical model of an agricultural truck is set up for the analysis of the nonlinear forces of two proposed Models under random road surface excitation which is implemented in MATLAB/Simulink platform. The obtained results indicate that the value of the root mean square (RMS) of the vertical acceleration response of vehicle body with Model 2 reduce by 5.11% in comparison with Model 1 when vehicle moves on ISO class D road surface at vehicle speed of 40 km/h and full load. Finally, both two hydro-pneumatic suspension struts are verified their ride comfort performances under various vehicle operating conditions and the obtained results also indicate that the performance with Model 2 significantly improved vehicle ride comfort in comparison with Model 1.