An experimental study was conducted to assess the acute oral toxicity of a phytogenic feed additives loaded herbosome formulation, following OECD (Organisation for Economic Co-operation and Development) toxicity guidelines 423. The formulation consisted of a scientifically developed combination of herbs, including Asparagus racemosus, Withania somnifera, Tinospora cordifolia, Phyllanthus emblica, Cinnomomum zeylanicum, Peuraria tuberose, Allium sativum, and piper nigrum in equal parts as poly herbal extract, combined with Phosphatidyl choline (1:3 ratio). Female Wistar Albino rats (n=6) were orally administered a dose of 2000 mg/kg body weight. Animals were observed for signs of toxicity, and body weight parameters were measured. No abnormal signs or symptoms were observed, and no mortality occurred during the study, indicating the safety of the formulation. The study, conducted in accordance with OECD guidelines 423, demonstrates the safe use of the phytogenic feed additives loaded herbosome formulation in Wistar rats. These findings suggest its potential as a safe and effective feed additive. Further investigations are required to explore its long-term effects and efficacy in different animal species. Overall, this study contributes to the understanding of the acute oral toxicity profile of the phytogenic feed additives loaded herbosome formulation.