Silent estrus is one of the most common problems in the buffalo farming industry. Quick and accurate estrus detection can reduce economic losses in the dairy sector. ‘Doka’ also referred as Temporary Engorgement of Teat (TET) is a behavioural pre-estrus sign which is characteristic of buffaloes. The present study was conducted with the objective to examine various endocrinological changes during ‘Doka’. The present study was conducted with the objective to examine endocrinological changes during ‘Doka’ in Murrah buffaloes. Non-pregnant lactating Murrah buffaloes (n=24) across parities maintained at ICAR-CIRB, Hisar in an organized dairy farm were divided into four groups with six animals in each group viz. Group I: Cyclic ‘Doka’, Group II: Cyclic without ‘Doka’. Group III: Acyclic ‘Doka’. Group IV: Acyclic without ‘Doka’. The present study confirmed that the cyclic ‘Doka’ and cyclic without ‘Doka’ animals had plasma progesterone levels of (>2ng/ml) while those in acyclic animals it was <1 ng/ml. The levels of plasma estrogen in ‘Doka’, cyclic, or non-cyclic animals across different groups were non-significant. However, ‘Doka’ showing animals have significantly high PGFM (13,14-dihydro-15-keto-PGF2alpha) concentrations (p<0.05) in comparison to cyclic without ‘Doka’ animals (4.32±0.29 vs 3.47 ±0.21ng/ml).