Cystolepiota is rarely studied in Pakistan, here we survey and describe new record of Cystolepiota from Himalayan moist temperate region of Pakistan. Originally, it was described from Europe. Cystolepiota moelleri Knudsen resembles with Western Asian collection in having free lamellae and non-dextrinoid spores. It differs in its rounded pileus and cylindrical cheilocystidia. The species is provided with full morpho-anatomical and phylogenetic description with line drawings of the microcharacters, discussion of related and similar species, and molecular data.