The core of veterinary anatomy is the study of three-dimensional animal body structures. The study of veterinary anatomy has traditionally served as the cornerstone of all disciplines in human medicine, veterinary medicine, and animal sciences. A classic anatomy lesson would involve didactic lectures utilizing chalk and talk, PPT presentations, the teacher doing a prosection, and the students performing a dissection. Creating 3D anatomical specimens, helps instructors and students to get rid of laboratories and museums which are filled with formalin specimens and their vapor. This study revealed a novel method for CT scanning and DICOM file generation for creating raw rasterized data. Additionally, the data were converted into STL (Standard Tessellation Language) files, allowing for simple 3D printing. The new technology was used to print an ox heart using Polyurethane(TPU) filaments through FDM and SLA-type 3D printers without sacrificing quality. Every person has the right to a healthy lifestyle. In this setting, 3D-printed teaching materials are a godsend for pedagogy. In this research, the materials, processes, and benefits of 3D printing of biological specimens for veterinary anatomy were examined. Creating veterinary anatomy specimens using 3D printing technology is undoubtedly the start of a new era in veterinary anatomy education.